FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25504330
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG444410.1742488432356388TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG67500.037.70043633
ACGTCTG73250.035.1334515
AGTCACG72650.034.93392628
CGTCTGA76000.033.77337616
TCGTATG72850.032.62327245
TTTCGGA77900.032.55083535
CTCGTAT73450.032.44914644
ACGTTTC79650.032.0327632
TTCGGAA79900.031.79311236
GTTTCGG82350.031.09374234
ATCGGAA85000.030.549692
GTCACGT84400.030.22997329
TCGGAAG86850.030.0025843
TCGGAAT88350.028.55011737
CACGTCT94200.027.58265514
CACGTTT97750.026.03283531
CACACGT100200.025.93099612
ACACGTC102350.025.51815813
CAGTCAC104100.024.85502627
GATCGGA109750.023.8495561
Produced by FastQC (version 0.11.5)