FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25465250
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG521070.2046200214017141TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG73050.034.18259415
AGTCACG73600.033.29038628
TCGTATG74300.032.38186345
CGTCTGA76900.032.29572316
CACGTGG79200.031.05076631
CTCGTAT79650.030.291744
GTCACGT81600.030.21915829
TCGGAAG86450.028.969483
ATCGGAA88750.028.06662
GCCTTAT88850.027.30111737
CACGTCT93700.026.76954314
ACGTGGC94000.026.28118732
GGCCTTA94200.026.01093736
TATCTCG95500.025.24001741
CACACGT102800.024.83748212
CAGTCAC101950.024.27569427
TCACGTG102150.024.27293230
CTTATCT100950.024.14344639
ACACGTC106500.023.76335313
CCTTATC107150.022.55559238
Produced by FastQC (version 0.11.5)