FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24385733
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG289580.11874976241230888TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG50050.031.78295528
ACGTCTG50750.031.60526515
CGTCTGA52450.030.4093216
TCGTATG52150.029.99306145
CGTGAAA55450.028.3628733
ACGTGAA56600.028.10426132
AACGATC51300.026.362338
CACGTGA60500.026.29328231
CTCGTAT51350.026.2530844
GAAACGA61500.025.64639736
GTCACGT62750.025.60096529
TCGGAAG64350.025.1070063
TGAAACG63050.024.94367235
CACGTCT66250.024.31284314
ATCGGAA67850.023.9445362
AAACGAT64950.023.83479537
CAGTCAC73500.022.19333327
CACACGT74350.021.84564412
TCACGTG75550.021.41288430
ACACGTC85100.019.27110513
Produced by FastQC (version 0.11.5)