FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26370442
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG303040.11491654178568565TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48200.030.85085115
CGTCTGA50950.029.09738216
AGTCACG52200.028.14613728
TCGTATG55050.026.817945
ACGTCCG55400.026.39832732
CTCGTAT57900.025.14836344
TCGGAAG60150.025.1019043
ATCGGAA64900.023.1606922
GTCACGT64650.022.89941829
CACGTCT68400.022.06902914
GTCCGCA66200.021.95624534
CCGCACA66150.021.90452236
TCACGTC74050.020.02360330
CACACGT77550.019.75519412
CAGTCAC76350.019.53776427
ACACGTC80050.019.19443513
CGCACAT79150.018.4218137
CGGAAGA89100.017.2489554
GATCGGA91200.016.6075951
TCCGCAC97050.015.62555835
Produced by FastQC (version 0.11.5)