FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29654449
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG522430.17617255339999743TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG82000.033.908615
CGTCTGA84400.032.97102716
TCGTATG84700.031.7264145
CTCGTAT87800.030.04244644
TCGGAAG97150.028.9982763
GTCCGCA95450.028.54633136
CACGTCT101950.027.49420414
ATCGGAA103750.027.0668142
CACACGT111750.025.1032112
CAGTCAC111300.024.88483227
ACACGTC116200.024.58713513
CGCATCT112450.023.71511539
AGTCACC119500.023.57248328
CCGTCCG120650.023.2925634
TCACCCG119350.023.13101630
CCCGTCC123200.022.38998433
ACCCGTC124700.022.21008532
TCCGCAT122400.022.17100337
CGGAAGA130200.022.0175574
CGTCCGC130050.021.83355735
Produced by FastQC (version 0.11.5)