FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28184624
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG413050.1465515381720189TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG75000.032.96396315
CGTCTGA78850.031.44002516
TCGTATG67800.030.97056445
CTCGTAT69800.029.95434244
TCGGAAG91350.027.16953
ATCGGAA92850.026.6578732
CACGTCT98250.025.6671914
CACACGT102850.024.34423612
GTCAGAA102300.023.99554836
GTCACAT102850.023.64787729
CAGTCAC106300.023.3883227
TGTCAGA105650.023.2555735
AGTCACA109550.022.53031728
ACACGTC113850.022.2094913
AATCTCG105250.022.0461241
TCTCGTA102750.020.5676243
ATGTCAG123100.020.2703534
GTCTGAA126700.019.90361617
GATCGGA125500.019.8342361
ACATGTC126050.019.74131832
Produced by FastQC (version 0.11.5)