FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30087115
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG798360.26534946936587306TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT107100.035.8476839
CCGTATC106900.035.807438
TCCGTAT108500.035.52647837
AGTTCCG110500.035.39003434
TCGTATG108600.035.33446545
ACGTCTG113650.035.23298615
CGTCTGA114350.034.99763516
CTCGTAT113000.034.11828244
TTCCGTA114900.033.72136736
TCGGAAG129550.030.7774583
TATCTCG126550.030.37593541
CACGTCT134400.029.97763814
ATCGGAA133550.029.8893322
GTCACAG134400.029.19493329
CACACGT141750.028.26454212
GTTCCGT138600.028.21415335
CAGTCAC141850.027.99510827
AGTCACA144450.027.38209528
TCACAGT146350.026.78138430
ACAGTTC147950.026.62809632
Produced by FastQC (version 0.11.5)