FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22896882
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG33050.024.91231315
CGTCTGA33150.024.769316
TCGTATG34900.023.2148345
CTCGTAT36750.020.76034744
TCGGAAG44400.018.852823
ATCGGAA45050.018.4309582
CACGTCT48600.017.21916414
CACAGTC48700.017.09444231
GTCACAG51950.016.06800829
CACACGT55650.015.44200112
CAGTCAC55400.015.23003427
TCACAGT57300.014.56811530
AGTCACA58500.014.38451128
ACACGTC63150.013.75052613
AATCTCG57950.013.47604941
ACAGTCA63150.013.46748232
GATCGGA68250.012.2342351
AGTCAAC70650.012.1974734
AGCACAC74850.012.08207510
GTCAACA69550.012.00180735
Produced by FastQC (version 0.11.5)