FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14859001
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT6050.026.0523722
AACCGCT2900.020.1743331
ATCGGAA8800.018.9344922
TCGGAAG8500.018.2770883
AGCGGTT5200.018.16758310
GTAGAAC1153.4642471E-617.60337411
TCCCTAC4450.017.1858149
GATCGGA9750.017.0914361
CGGAAGA9000.017.010324
TTTCCCT6300.016.7844267
GTGTAGA2301.8189894E-1216.63201530
TAGATCT2301.8189894E-1216.63145433
GAGTGTA2057.4578566E-1116.46481128
TTCCCTA5200.016.4366198
AGTGTAG2201.2732926E-1116.3651929
TCGTGTA3150.015.70932514
GTAGATC2302.5465852E-1115.65366132
TACACGA4850.015.30444613
CGGTTCG4300.015.16983212
CATTCCT4050.015.00162620
Produced by FastQC (version 0.11.5)