FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29032691
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40450.012.28952911
CGTGACT30000.011.6334642
GTACTCG62750.011.4452724
TCAGGTA52350.010.3998597
AGTACTC75000.010.026883
TACTCGA70350.010.016435
GCGTGAC39500.09.5205861
GAATCGA42400.09.345364
TAGAACA56050.08.98946612
GGAAAAT143600.08.8914571
CAGGTAG60800.08.8786148
TCACACG29600.08.73863615
CTCGAAT25600.08.4445311
GGTAGAA62150.08.28810110
AAAATCC101000.08.2259423
AATTGAC51050.07.98207045
GTGACTC50000.07.970473
TCCGTTA22150.07.92445519
GAATTGA76250.07.8540014
CACTCGT57300.07.69518535
Produced by FastQC (version 0.11.5)