FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27281580
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC49500.013.35992911
CTAATCG34450.012.8098223
TAATCGA41600.011.9603774
TAGAACA62550.010.82433512
TCAGGTA67550.010.6573197
CAGGTAG67950.010.1291398
GGTAGAA68350.09.93870210
AATCGAG64800.09.7972315
GGAAAAT149150.09.0889571
AAAATCC106050.09.0443043
CATAAGC138150.09.0215727
AGCATAA135700.08.859125
GCTAATC57750.08.8115882
GGTAAGT21550.08.66383917
AGGTAGA79800.08.4844729
GAATCGA40100.08.30927854
GCATAAG147150.08.1490216
ACCGAAC37100.08.12441510
AGATCAG114350.08.1116094
TAAGCAA157500.08.0548939
Produced by FastQC (version 0.11.5)