FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24177861
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36250.010.36262311
CGTGACT19250.09.9420812
GTACTCG39200.08.95949654
TCCGTTA33100.08.70227519
GGAAAAT100700.08.6093391
ACTTTCG18350.08.58445719
CTCGAAT21800.08.4702431
CACTCGT47850.08.4627635
TCAGGTA49100.08.3849767
TAGAACA47700.08.29956912
ACTCGTG49750.08.22826236
GCGATTT45000.08.0065611
CGTTAAC36150.07.96805921
TAAGTCT17250.07.96185973
AAAATCC73900.07.95188143
TTAAGTC19950.07.9003122
GCGGAAA60150.07.89924141
GAATCGA36300.07.87666034
AATCGAA45900.07.84753855
TCACTCG51200.07.82116634
Produced by FastQC (version 0.11.5)