FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24569805
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC31150.010.10953211
CGTGACT20600.09.1814062
GTACTCG37350.09.1019574
CACTCGT55050.08.9905235
GGAAAAT95450.08.6112161
ACTCGTG59650.08.59697536
CTCGAAT21800.08.5737181
TCACTCG61550.08.18727534
GCGGAAA69950.08.1447981
CTCTATA61600.08.10798545
AAAATCC74650.08.0228653
GCGTGAC27650.07.8999471
GGGGAAT52750.07.89763071
TTACGAC21150.07.873136529
AATCGAA46200.07.79661565
TACTCGA43650.07.5816065
TACGGTA21700.07.568640740
GGACTTA35700.07.5676584
GAATTGA66400.07.56110434
AGCCTAG24450.07.543165725
Produced by FastQC (version 0.11.5)