FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24876649
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23050.012.6010772
GTAGAAC37850.011.70727811
GTACTCG51550.011.5296494
AGTACTC65750.09.8277883
TACTCGA60600.09.7701895
GCGTGAC33200.09.5635061
TAGAACA48200.09.24003812
GAATTGA59800.08.7719384
GGTAGAA51800.08.7715510
AAAATCC86800.08.6116833
TCAGGTA53800.08.5305297
ACTCGTG45600.08.43445936
ATTGACC38950.08.4330866
CACTCGT45000.08.39872735
GTGACTC38950.08.2660543
GGAAAAT111500.08.2600851
AATTGAC40550.08.2165735
AAAAATC69700.08.10832
ACACGCG22200.08.0059956
CTCTATA46900.07.91507345
Produced by FastQC (version 0.11.5)