FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27064042
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25800.012.567282
CGTGTAG16550.011.68705716
GTACTCG55650.011.5300814
GCGTGAC34500.010.7698161
GTAGAAC33150.010.44954111
AGTACTC65750.010.2732123
TACTCGA65350.09.5425485
GGAAAAT112450.09.3318961
ACACGCG24250.08.8137556
CTCTATA61050.08.62319345
CACTCGT59600.08.60621335
GTGACTC43050.08.4204153
TCGTGTA23550.08.40477615
AAAATCC80700.08.3979593
ACTCGTG63500.08.21762336
TTAAGTC25300.08.1877732
GTAGATC26750.08.07557932
GCGGAAA71700.08.0715591
GAGTGTA24550.08.06589528
TCACTCG65900.07.988291734
Produced by FastQC (version 0.11.5)