FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25107492
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26600.013.3740222
GCGTGAC34300.011.8170281
GTAGAAC34650.010.32186211
GTACTCG51600.010.2531454
CTCGAAT20900.09.4812641
AGTACTC59250.09.3478873
GGAAAAT100800.09.3378231
GTGACTC42200.09.1766193
TACTCGA58250.08.966295
AAAATCC76850.08.7304983
GAATTGA62650.08.6244044
AATCGAA47500.08.5309945
ACACGCG23950.08.2664376
GAATCGA34550.08.2103464
CACTCGT60250.07.990622535
CTCTATA60250.07.95350545
GCTCTAG48500.07.930828611
ACTCGTG60600.07.905663536
TAGAACA45200.07.86290112
GAAAAAT124300.07.84416341
Produced by FastQC (version 0.11.5)