FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24225799
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT20900.012.8204952
GCGTGAC28400.010.1495531
TCGGGGG67800.09.72301839
TTTCGGG30000.09.44965737
GTACTCG47700.09.2038814
GGAAAAT113300.09.0832561
CTCTATA54450.09.00728745
GTAGAAC30000.08.99733311
ACTCGTG54300.08.94703336
CACTCGT54650.08.89173235
ATTTCGG24000.08.80930636
AAAATCC79600.08.5989913
AGTACTC55300.08.5095823
GAATTGA64200.08.2411454
AAAGGGG86450.08.19537526
TCACTCG58500.08.19118134
AATTGAC41650.08.1619895
AATTCAC31950.08.1032855
GGGGGAA112650.08.09044221
TTCGGGG63750.07.97618138
Produced by FastQC (version 0.11.5)