FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n02_E1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n02_E1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24089331
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG22900.021.50862716
GAGTGTA28850.017.15935528
TCGTGTA29600.016.4894915
GTAGATC33950.015.17846832
ATCGGAA54750.015.0513833
TGTAGGG34100.014.9139818
TCTCGGT35950.014.26908237
GTGTAGA36200.014.23484730
AGTGTAG34750.014.11651929
GTGTAGG35600.013.90111917
TAGGGAA39900.013.53572120
TAGATCT37700.013.48960833
ATCTCGG43750.013.10971536
GTAGGGA41200.013.10862319
TCGGGGG87500.012.85629239
TCGGAAG53050.012.6043334
GATCGGA74550.012.5644162
ATTTCGG26350.012.29172936
TTTCGGG34650.012.27209237
TTCGGGG77150.011.22754738
Produced by FastQC (version 0.11.5)