FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14859001
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA5650.038.21392416
ACGTCTG5800.037.22563615
CACACGT6300.034.62425212
CACGTCT6750.032.98682814
ACACGTC7100.030.7224113
CAGTCAC5550.028.79516627
GCACACG8150.027.31660811
CCAGTCA6950.026.55010826
TCGGAAG10400.025.7457493
AGCACAC8300.025.73918210
ATCGGAA10550.025.1639612
CGGAAGA10950.024.4525814
GATCGGA11350.023.1964421
CTCCAGT9600.021.79800424
AACTCCA9950.021.70893922
GAACTCC10950.019.93072921
CTGAACT11450.018.66437719
TGAACTC11900.017.96269420
AGAGCAC12250.017.6248468
TCCCTAC2800.015.27137440
Produced by FastQC (version 0.11.5)