FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29032691
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG443810.1528656093229525TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG69500.031.91319515
CGTAATC66850.031.20569438
CGTCTGA71500.030.92613616
CCGTACG71800.030.27490233
TCGTATG72850.029.62543745
CTCGTAT72150.029.47754944
ACCGTAC78450.028.1102532
CACCGTA81900.026.73415631
ATCGGAA84950.026.0633982
TCGGAAG87100.025.5235543
TACGTAA84300.025.41032636
CACGTCT92300.024.5422814
ACGTAAT87750.024.2075337
GTAATCT89350.023.44979539
CACACGT99750.022.61921112
CAGTCAC100050.022.3122127
AATCTCG95950.022.25966541
ACACGTC106800.021.31554413
TCTCGTA103150.020.9022843
AGTCACC109000.020.50072728
Produced by FastQC (version 0.11.5)