FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27281580
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG301520.11052145806804445TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG46950.033.4070533
ACGTCTG55300.029.2868315
AGTCACG54650.028.86629928
CGTCTGA58600.027.75272816
CTCGTAT54900.026.44147344
TCGTATG55500.026.31676145
ACGTTTC59950.026.23832532
TTTCGGA60250.026.0301635
GTTTCGG61300.025.84109534
TTCGGAA61800.025.30527136
GTCACGT65000.024.30411529
ATCGGAA66700.024.2545832
TCGGAAG69850.023.451043
TCGGAAT71750.021.76555637
CACGTCT82200.020.38726814
CACACGT81300.020.1705212
ACACGTC83950.020.14988113
CACGTTT81450.019.50606531
CGGAATC78700.019.3583938
GATCGGA91550.018.017171
Produced by FastQC (version 0.11.5)