FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24177861
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG315600.13053263892947353TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG51200.031.32513815
AGTCACG50550.031.11884528
TCGTATG52350.029.74824745
CGTCTGA55450.028.7213716
CTCGTAT56400.027.85222844
CACGTGG56950.027.77945131
TCGGAAG63250.025.4001853
GTCACGT62850.025.10001229
ATCGGAA63850.025.0909652
GCCTTAT66150.023.91386837
CACGTCT67050.023.88686414
ACGTGGC68300.023.2942732
TATCTCG68200.023.0332241
GGCCTTA70550.022.51727736
CACACGT74250.021.78308112
CAGTCAC77000.020.92621427
CTTATCT76250.020.86637539
CCTTATC77800.020.13134838
ACACGTC81850.019.97999813
TCACGTG80700.019.7712830
Produced by FastQC (version 0.11.5)