FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24569805
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48600.029.11304915
AGTCACG47900.029.08131828
CGTCTGA50050.028.22466916
TCGTATG49450.027.71592545
CTCGTAT48550.025.26381344
ACGTGAA57000.024.43766632
CGTGAAA56850.024.42223233
CACGTGA58400.023.96786931
TGAAACG59650.023.31147635
AACGATC52700.023.14373438
GTCACGT62450.022.41350729
CACGTCT64700.022.25124414
TCGGAAG64000.022.1142923
GAAACGA64600.021.45595736
ATCGGAA67000.021.157512
AAACGAT66750.020.59711837
CACACGT71000.020.3722612
ACACGTC77700.018.90478113
CAGTCAC75450.018.61173827
TCACGTG80550.017.62851330
Produced by FastQC (version 0.11.5)