FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24876649
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG34150.024.3051615
TCGTATG37300.022.56521645
CGTCTGA37600.022.13485316
AGTCACG37400.021.96305528
ACGTCCG39550.020.99578732
CTCGTAT40000.020.93021444
GTCCGCA42750.019.10641334
ATCGGAA47300.017.6480582
TCGGAAG49200.017.2410583
GTCACGT48400.017.20348729
CCGCACA49350.016.5973136
CACGTCT53250.016.39014414
TCACGTC54950.015.19382930
CACACGT56950.015.1279212
CAGTCAC63300.013.97222527
CGCACAT59450.013.89174737
TCCGCAC66000.013.29641435
ACACGTC67800.012.97234813
TCTCGTA73500.011.51314243
GATCGGA77650.011.215311
Produced by FastQC (version 0.11.5)