FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27064042
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG573600.21194173434995406TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA87750.033.5812416
ACGTCTG88050.033.5434715
TCGTATG85250.032.81239745
CTCGTAT88400.031.51682344
TCGGAAG101750.029.3008373
ATCGGAA102200.029.0396692
GTCCGCA99700.028.88858236
CACGTCT105950.028.02577414
CACACGT114500.026.16930612
CAGTCAC114000.025.91921627
CGCATCT113400.024.90576439
AGTCACC122600.024.30293528
ACACGTC124500.024.28368213
CCGTCCG122000.024.21610534
ACCCGTC123700.023.68553532
TCACCCG126500.023.14395130
TCCGCAT123100.023.1421237
CCCGTCC126900.023.10612533
CGTCCGC129000.023.0067535
CGGAAGA133200.022.686654
Produced by FastQC (version 0.11.5)