FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25107492
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG274900.10948923134178436TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG53450.030.04857315
CGTCTGA54000.029.70086316
TCGTATG47850.028.17202445
CTCGTAT50850.026.1126344
TCGGAAG65350.024.996963
CACGTCT66800.024.71752714
ATCGGAA66550.024.3770772
GTCAGAA68900.022.92584236
CACACGT73500.022.34229912
GTCACAT73950.021.78810529
TGTCAGA76700.020.9457335
AGTCACA78000.020.7148728
CAGTCAC78750.020.4320227
AATCTCG73350.020.0049341
ACACGTC84450.019.97777213
ATGTCAG92900.017.68068134
CGGAAGA95350.017.3681724
AGCACAC98250.017.26362410
GATCGGA97000.017.144511
ACATGTC97800.017.00352532
Produced by FastQC (version 0.11.5)