FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24225799
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG781840.32273032563342907TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT103250.036.4395337
CCGTATC103100.036.3203438
TCGTATG103600.036.1255645
CGTATCT103850.035.97410239
CTCGTAT105300.035.52250744
AGTTCCG108050.035.4637834
ACGTCTG112150.035.11958315
CGTCTGA113900.034.50116
TTCCGTA113750.033.15384736
ATCGGAA124450.031.784052
TATCTCG119550.031.30715841
CACGTCT128200.031.03950914
TCGGAAG127700.031.0106353
GTTCCGT123700.030.66814635
CACACGT134200.029.53472112
GTCACAG131200.029.48455429
CAGTCAC134700.028.65261727
ACAGTTC135050.028.39359532
AGTCACA136500.028.35680628
ACACGTC141300.028.11400213
Produced by FastQC (version 0.11.5)