FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l05_n01_E1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l05_n01_E1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24089331
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1312510.5448511625333223TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG174250.039.8180645
ACGTCTG186850.038.18661515
CGTCTGA187600.038.06992316
CTCGTAT176700.036.6693744
ATCGGAA200000.035.6751862
TCGGAAG200900.035.560393
CACGTCT204350.034.9943414
CACAGTC205200.034.43485631
CACACGT210300.034.0155812
GTCACAG210200.033.637129
CAGTCAC212100.033.44298627
ACACGTC216400.033.08743313
AGTCACA214400.033.02083228
TCACAGT215650.032.6932330
AATCTCG203150.032.1278941
AGTCAAC220050.032.11705834
ACAGTCA220700.032.0974332
GATCGGA224100.031.8836271
CGGAAGA231800.031.0044614
GTCAACA228400.030.8740735
Produced by FastQC (version 0.11.5)