FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14090766
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT5700.028.4422072
AACCGCT3050.018.44337531
GAGTGTA2300.017.60834928
AGTGTAG2200.017.38707229
TTCCCTA4250.016.9321868
TACACGA3350.016.78493513
TCCCTAC3500.016.7060689
TCGGAAG7300.016.3467883
GTAGATC2403.6379788E-1215.93820532
AGCGGTT5100.015.87674210
TGAACCG3550.015.84571529
TCTCGGT2453.6379788E-1215.61210437
ATCGGAA8050.015.1044032
GTGTAGA2701.8189894E-1215.0006130
CGGTTCG3900.014.9945412
TAGATCT2601.0913936E-1114.71203233
TTTCCCT5850.014.6111037
GATCGGA8700.014.2348781
CCCTACA4350.013.96058410
CGGAAGA8650.013.7945754
Produced by FastQC (version 0.11.5)