FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28838062
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTCG58800.012.24806319
CGTGACT22500.010.9088372
ATAGGGG67050.010.63673534
GTACTCG49950.010.5480994
GCCCTAT70600.010.44921510
TGGTAGG69100.010.32163827
TACTCGA50900.010.2160015
TGCCCTA74950.010.1417319
GATAGGG71400.010.02109433
CGCGATA20700.010.00012129
GGATAGG74300.09.78142732
GGAAAAT123300.09.4238891
TAGGATA75450.09.33410830
AGTACTC62750.09.32978253
ATTAGGG67600.09.3176676
GTAGAAC32550.09.25915311
TTAGGGT67550.09.2572547
GGTAGGA82950.09.24911528
TTAAGTC36600.09.2287882
AAAATCC93700.09.1317893
Produced by FastQC (version 0.11.5)