FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30990476
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG44950.012.924253
TAATCGA50750.012.6445614
GTAGAAC54000.010.91272711
AATCGAG85650.010.4865285
GCTAATC68400.09.5142812
GGAAAAT154050.09.4139161
AAAATCC118750.09.3292513
GGTAAGT35800.09.11095717
GGTAGAA69750.08.60983710
TAGAACA69000.08.60556112
AAAAATC101950.08.5257842
TCAGGTA75650.08.2663587
CGAAATT51100.08.196462
AATTGAC53450.08.17034155
CGTAACG39500.08.14531242
AATCGAA67500.08.1371495
AAATCCT125150.08.1320964
GAATCGA48050.08.0599074
ACTTTCG25250.08.02193219
CAGGTAG74600.07.9885058
Produced by FastQC (version 0.11.5)