FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23742892
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC32500.010.03488111
CGTGACT18500.09.0070172
GTACTCG33400.08.8307434
CACTCGT44900.08.31797135
GGAAAAT96850.08.1841071
GCGGAAA58800.08.080411
AAAAATC75100.08.0655372
ACTCGTG49100.07.78811836
GAATTGA67650.07.6880584
TACTCGA37900.07.66164885
CTCTATA49300.07.529786645
TCAGGTA46250.07.48986677
AATTGAC40900.07.42988635
TCACTCG51100.07.352774634
AATCGAA48550.07.232815
CTCGAAT23150.07.19795371
CTATAAG26350.07.17746544
TAGAACA46950.07.13801312
GGTAGAA45400.07.133988410
GAAAAAT132400.07.07511761
Produced by FastQC (version 0.11.5)