FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23080806
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC29200.011.2457211
ACTTTCG31650.010.31092819
ATTAGGG40650.09.9612096
GTACTCG35650.09.7895374
AATCGAA45350.09.6295735
TTAGGGT38850.09.6113467
ACATGCG32800.09.6018366
GGAAAAT96300.09.4706331
GCCCTAT38950.09.29690710
TACTCGA37250.08.9437165
CGTGCGA32700.08.80398911
CTCGAAT20800.08.7694421
AAAATCC78950.08.7274723
TGCGAGT32150.08.67480813
TGGTAGG41950.08.58133727
CGTGACT18350.08.4674742
GCGATTT77750.08.3414661
CATGCGT35900.08.3334417
AAAAATC64150.08.319412
CACTCGT42050.08.2929735