FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28601316
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29450.013.9921312
GCGTGAC37100.011.7141461
GTAGAAC37200.011.1857511
GTACTCG60050.011.1730354
TACTCGA60700.010.5687525
AGTACTC72950.09.5681133
GTGACTC46500.09.3453233
GGAAAAT120950.09.085341
AATTGAC48650.08.60592655
TCAGGTA52150.08.5835217
GCTCTAG55750.08.43213811
CTAGCAC50300.08.3620414
AATTCAC43200.08.1805695
CTATAAG33450.08.1443634
TAGAACA51450.08.13131612
GCGGAAA63050.08.0714311
CACTCGT50550.08.05585235
CCCGAAT17800.07.9698061
AAAATCC92300.07.87935733
CGATAGT14600.07.859733631
Produced by FastQC (version 0.11.5)