FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29139486
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26100.012.8550982
ACTTTCG43700.012.61780319
GTACTCG51250.011.4664384
ATAGGGG51700.011.14015334
GTAGAAC32900.010.87017111
GCCCTAT55400.010.67767310
TACTCGA54200.010.6736275
TGGTAGG54500.010.48572227
GATAGGG55200.010.43482433
TGCCCTA62250.09.8267249
TAGGATA60100.09.50922230
TTAAGTC40650.09.4725122
AGTACTC65800.09.3420093
GGAAAAT125600.09.2869741
GCGTGAC39050.09.2840811
AGTCTCG36700.09.2616645
CTATAAG31850.09.2606984
GAACGCT35450.09.1421521
GGATAGG63750.09.03536132
TCCGTTA100300.08.97549119
Produced by FastQC (version 0.11.5)