FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29165833
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27100.012.7132132
GTACTCG53500.010.9844464
GTAGAAC36150.010.2660211
GCGTGAC37700.010.1541891
TACTCGA57350.09.8912515
GGAAAAT129600.09.5042961
AAAATCC94450.09.34543
AGTACTC67600.08.7936873
AATCGAA60450.08.7881975
TAAATCG10650.08.6685813
CTCTATA65300.08.44114845
ACTCGTG63100.08.41294136
GAATCGA46050.08.3120994
AAATCCT97850.08.2608564
CACTCGT65250.08.1373135
GCGGAAA80050.08.10156251
GTGACTC48100.08.0518773
GAATTGA82100.08.0355544
TCACTCG69350.07.72111234
AATTCAC46650.07.67236955
Produced by FastQC (version 0.11.5)