FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29427190
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG62750.011.6255314
CGTGACT23750.011.5670382
TACTCGA64500.010.9932475
GTAGAAC41750.010.98999111
AGTACTC78200.09.9051063
AAAATCC103000.09.2690673
AATTGAC52000.09.2204365
GCGTGAC33450.09.0881161
GAATTGA77900.09.017764
GGAAAAT139150.09.0138061
TATAAGG31900.08.7499625
CTATAAG31300.08.4882714
TAGAACA53900.08.47091212
TCAGGTA57500.08.4499017
GGTAGAA57550.08.24640210
AAATCCT107750.08.1285654
CACTCGT53700.08.04403435
AATCGAA60100.07.9777485
GAATCGA44050.07.97370624
AAAAATC84300.07.90660432
Produced by FastQC (version 0.11.5)