FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n02_D1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n02_D1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23911705
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22850.011.8255862
GCGTGAC29400.011.10594751
GTACTCG45850.010.4107134
TACTCGA48000.09.9886515
GTAGAAC29200.09.55141111
AGTACTC56700.09.0940893
AAAATCC78750.08.8351653
GGAAAAT110900.08.7108531
CTCGAAT23200.08.1531651
GAATTGA69050.08.1519284
GAATCGA36350.08.1142974
GTGACTC36650.08.10973553
AAATCCT82850.07.96267754
AAAAATC65150.07.6730182
TCCGTTA32850.07.604787319
ACTCGTG45000.07.59784836
GAAAATC93000.07.481752
TAGAACA38650.07.448832512
GAAAAAT116400.07.44805151
CTCTATA45700.07.433851245
Produced by FastQC (version 0.11.5)