FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14090766
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG4850.035.22996515
CGTCTGA5100.033.94383616
CACGTCT5300.032.2491614
CACACGT5750.030.11472312
ACACGTC5950.029.48041713
CCAGTCA5550.026.76760326
AGCACAC6700.025.50907910
TCGGAAG9400.024.6411363
CAGTCAC5650.024.3019727
ATCGGAA10050.023.2694592
CGGAAGA10450.022.1649154
GCACACG7950.021.7810911
GATCGGA10850.021.7637651
CTCCAGT8300.019.25424224
GTCCTAT1601.607259E-816.88394243
AGAGCAC10600.016.548978
AACTCCA9850.016.224122
AGTCCTA1806.845403E-815.00794742
TAAGGTG1951.12413545E-815.00618728
CTGAACT12300.014.6223119
Produced by FastQC (version 0.11.5)