FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28838062
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49700.027.47544715
CGTCTGA51700.026.36905316
CCGTACG50500.026.1104533
CGTAATC50100.025.3803738
TCGTATG50950.024.82167645
CTCGTAT51800.024.3286744
TCGGAAG60600.022.7232063
ATCGGAA61950.021.8649752
CACGTCT69400.019.8716114
TACGTAA67150.019.4022136
CACACGT74000.019.24351712
ACCGTAC73300.018.94001232
CAGTCAC73950.018.80383127
GTAATCT68800.018.67806239
ACGTAAT70700.018.49266437
CACCGTA71850.018.38239731
ACACGTC81250.017.5540913
AGTCACC84150.016.76537328
TAATCTC77100.016.75492340
AATCTCG85100.015.60224341
Produced by FastQC (version 0.11.5)