FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30990476
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG446270.1440023057406411TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG68850.035.58984833
ACGTCTG74200.033.74439215
AGTCACG74950.032.69285228
CGTCTGA80650.031.10146716
ACGTTTC79700.030.71617732
TCGTATG76450.029.75863345
TTCGGAA81550.029.57933636
CTCGTAT76650.029.38878344
TTTCGGA83600.029.31110635
ATCGGAA86250.028.9836882
GTCACGT85200.028.62805729
TCGGAAG89300.028.27073
GTTTCGG89150.027.5619434
TCGGAAT88400.027.16188437
CACACGT96450.026.28679712
CACGTCT102400.024.6723514
ACACGTC103750.024.41553713
CACGTTT101750.024.1701631
GATCGGA110700.022.6667391
CGGAATC105850.022.36732138
Produced by FastQC (version 0.11.5)