FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23742892
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG407820.17176509079011942TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG61700.032.7783615
AGTCACG62350.031.9014828
CGTCTGA66600.030.36673716
CACGTGG66050.030.21617131
TCGTATG65100.029.96983545
CTCGTAT66950.029.04158644
GTCACGT71550.027.83128229
TCGGAAG73700.027.4466323
GCCTTAT73200.027.23737137
ATCGGAA74300.027.1947612
CACGTCT75850.026.9913214
TATCTCG73950.026.50579341
GGCCTTA76400.026.30122636
ACGTGGC79300.025.5367332
CACACGT83900.024.61515212
ACACGTC87700.023.85641513
TCACGTG86500.023.17696630
CTTATCT88850.022.44149639
CCTTATC88750.022.41620838
CAGTCAC92750.022.07601427
Produced by FastQC (version 0.11.5)