FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23080806
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG39500.028.76686728
ACGTCTG41250.027.86721815
TCGTATG41500.027.05877545
CGTCTGA44200.026.00730116
CTCGTAT39400.025.36037344
CGTGAAA45050.024.77401733
ACGTGAA46250.024.2765632
CACGTGA48050.023.55393431
GTCACGT49750.023.11149229
TCGGAAG50400.022.9017243
TGAAACG50000.022.50203535
ATCGGAA51000.022.4117032
AACGATC46450.021.51234638
CACGTCT55550.021.14067514
TCACGTG55150.020.88933630
AAACGAT54850.020.18567337
CACACGT57500.020.11011312
GAAACGA57300.019.439136
CAGTCAC63800.018.09211527
ACACGTC66800.018.01758413
Produced by FastQC (version 0.11.5)