FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28601316
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA50150.028.5740316
ACGTCTG50350.028.41584815
AGTCACG49700.028.2957728
ACGTCCG54200.025.69776332
CTCGTAT55100.025.15898344
TCGTATG57100.024.1583845
GTCCGCA62200.022.5743734
ATCGGAA63900.022.3598292
CCGCACA63450.022.20049536
GTCACGT64400.022.1169729
TCGGAAG65950.021.6988353
CACGTCT71950.020.38635314
CACACGT76050.019.61213512
TCACGTC74000.019.06519730
CGCACAT75350.018.42705737
TCCGCAC83350.017.65611335
ACACGTC85900.017.57275813
CAGTCAC82750.017.48378827
TCTCGTA92950.015.18038743
GATCGGA97750.014.8487611
Produced by FastQC (version 0.11.5)