FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29139486
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG293020.10055771059242433TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG52300.029.98006415
CGTCTGA53950.029.5218316
CTCGTAT56300.026.6615844
TCGTATG57150.026.30337745
TCGGAAG65400.024.3234023
ATCGGAA65000.024.1616742
CACGTCT71800.022.46562614
GTCCGCA69300.021.95073536
CACACGT76850.021.33990912
CAGTCAC82000.019.59204327
CCGTCCG82450.019.37728334
ACACGTC86100.019.04729513
CGCATCT80700.018.65725939
AGTCACC90000.018.15072428
AGCACAC96800.017.12777910
CGTCCGC95000.017.10179735
GATCGGA94350.016.957851
CCCGTCC102000.015.72933
TCTCGTA97050.015.69864943
CGGAAGA105150.015.5776454
Produced by FastQC (version 0.11.5)