FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29165833
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG348750.11957484636218002TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG70100.030.197815
CGTCTGA71250.029.83668916
TCGTATG61400.028.66048645
CTCGTAT63200.027.2758644
TCGGAAG80950.026.4613723
ATCGGAA82300.025.972772
CACGTCT88300.024.45896114
GTCACAT96650.022.11683529
CACACGT99650.021.9883212
GTCAGAA96550.021.88462636
CAGTCAC98250.021.84770827
TGTCAGA97450.021.6361435
AATCTCG91750.020.84894641
AGTCACA104050.020.6516328
ACACGTC107900.020.26540213
GATCGGA115150.018.6444551
CCAGTCA120450.017.7835626
CGGAAGA122000.017.7789344
AGCACAC125550.017.64939910
TCTCGTA99850.017.53469843
Produced by FastQC (version 0.11.5)