FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29427190
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG424770.14434609624636263TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT66700.031.4124639
AGTTCCG70000.031.08486634
TCCGTAT67950.031.06406437
TCGTATG68950.030.4506145
ACGTCTG74300.030.00481615
CCGTATC71100.029.5637738
CTCGTAT71350.029.3640144
CGTCTGA76300.029.15935316
TTCCGTA77300.027.8289936
GTTCCGT85150.025.47496835
TCGGAAG90400.024.7155973
ATCGGAA90500.024.4646512
TATCTCG85050.024.44899641
CACGTCT97400.023.46733714
CACACGT100650.022.55241812
TCACAGT100150.021.7033530
GTCACAG101350.021.60189829
CAGTCAC104650.021.13511327
ACACGTC111900.020.58664713
ACAGTTC108450.020.20837832
Produced by FastQC (version 0.11.5)