FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l04_n01_D1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l04_n01_D1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23911705
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG275770.11532845524817238TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48950.030.2397715
CGTCTGA50700.029.41784716
TCGTATG49800.028.920545
CTCGTAT48300.027.25730944
TCGGAAG62600.023.830073
ATCGGAA62500.023.6522942
CACAGTC61850.023.64626331
CACGTCT68300.022.13498714
CACACGT69700.021.43122312
CAGTCAC71000.021.16963227
GTCACAG71650.020.66380929
AGTCACA72150.020.48916228
TCACAGT73250.019.81298830
ACACGTC78050.019.51315113
AATCTCG71350.018.51488741
ACAGTCA82600.018.25117732
AGCACAC89500.017.31840110
GATCGGA86200.017.282331
GTCAACA86350.017.27695835
TCTCGTA78050.016.95425243
Produced by FastQC (version 0.11.5)