FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13696196
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT5750.025.0648782
GTAGATC3100.022.49842332
GTGTAGA3000.022.49842330
AGTGTAG3100.021.77234829
TCTCGGT3400.021.1766137
GAGTGTA3200.021.09126928
TAGATCT3500.020.56998633
GTGTAGG4000.017.99604417
TCCCTAC3650.017.8723269
TCGTGTA4050.017.77309215
AACCGCT2750.017.18061431
AGCGGTT5100.016.7605310
TGTAGGG4200.016.60834318
TGTAGAT4250.016.41061431
TAGGGAA4700.016.27842520
GATCTCG4600.016.13905335
TTCCCTA4600.016.1372288
GTAGGGA4750.016.10707519
ATCGGAA8100.015.0128192
TTTCCCT6000.014.999777
Produced by FastQC (version 0.11.5)