FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32416010
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG62400.011.0055914
GTAGAAC38850.010.71168711
TACTCGA66300.010.0502865
AGTACTC74550.09.6963843
GGAAAAT157250.09.6666871
CTCGAAT32100.09.6112541
AAAATCC118050.09.2327653
AATCGAA71200.09.0424535
CGTGACT26150.08.6979092
GAATCGA53650.08.6875784
GAATTGA91800.08.0941154
AAATCCT116650.07.9333244
GAAAATC133700.07.9164712
TAGAACA53450.07.8699112
AATTCAC47800.07.81772575
ACGGTAC15600.07.789408720
AAAAATC94200.07.62614352
CTCTATA60950.07.603183345
TTAAGTC25900.07.56457762
ACACGCG30000.07.5002876
Produced by FastQC (version 0.11.5)